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The FBI has now stepped in to assist local authorities. The Bureau’s Operational Technology Division released new details about a suspicious individual captured on doorbell camera footage outside Guthrie’s residence.
The individual seen in the black-and-white footage is described as standing between 5 feet 9 inches and 5 feet 10 inches tall with an average build. Investigators say the person was wearing gloves and a ski mask and carrying a 25-liter Ozark Trail Hiker Pack backpack. The image, though grainy, has become a key focus for federal and local investigators working to identify a possible suspect.
Perhaps the most significant development came from Pima County Sheriff Chris Nanos, who revealed that forensic teams have recovered genetic material from the scene that does not belong to Guthrie.
“What I believe is what I know is we found DNA,” Nanos told Fox News correspondent Jonathan Hunt during a sit-down interview.
When pressed further about whether the biological evidence matched the missing woman, Nanos made the situation even more clear.
“That was not Nancy Guthrie’s DNA.”
Authorities are now working with a contracted forensic laboratory in Florida to process and analyze the material. According to Nanos, this move follows standard investigative protocol, despite critics questioning how the evidence has been handled.
The sheriff emphasized that investigators are gathering elimination samples from individuals connected to the home in order to rule out known sources. The goal is to narrow down any potential matches and determine whether the recovered DNA could belong to a suspect.
Nanos also clarified confusion surrounding a glove that has been discussed in connection with the case. The item was not found at Guthrie’s residence. Instead, it was discovered roughly two miles away during expanded search efforts, suggesting that investigators are casting a wide net.
Despite the mounting concerns, Nanos insisted the department remains focused on bringing Guthrie home safely.
“They all have hope and belief that this is a rescue mission,” he said.
Law enforcement agencies have deployed ground search teams, aerial units, and federal partners in a coordinated effort to retrace Guthrie’s last known movements. Investigators are combing through residential security footage from nearby homes and mapping every possible route connected to her disappearance.
Officials say forensic testing remains ongoing, and while early lab results have provided few definitive answers, the investigation is far from stalled.
Family members have publicly pleaded for information, urging anyone who may have noticed unusual vehicles, unfamiliar faces, or suspicious activity in the days leading up to Feb. 1 to come forward.
Authorities continue to stress that the case is active and expanding.
What began as the disappearance of an elderly Tucson woman has now evolved into a multi-agency operation involving SWAT teams, federal agents, forensic labs, and multiple detentions.
And with unidentified DNA at the center of it all, the stakes have only grown higher.
The central question remains: whose DNA was found inside Nancy Guthrie’s home—and what does it reveal about what truly happened?
